Embryogenic cell suspensions and plant regeneration through somatic embryogenesis in banana and plantain Musa spp.
1992
Dhed'a, D. (Kisangani Univ. (Zaire). Faculte des Sciences)
Embryogenic cell suspensions have been initiated using explants from meristematic shoot-tips (scalps). The culture medium has been a modified Murashige and Skoog medium supplemented, according to the steps of culture, with 5 microM 2,4D, 1-10 microM BAP or zeatin. The suspensions obtained for 5 banana varieties have regenerated plants through somatic embryogenesis. Embryogenic cell suspensions have proved to be the material of choice for cryopreservation, protoplast isolation and culture and for genetic manipulation of Musa for resistance to diseases.
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