Absorption of sucrose by tissues of adult tubers of potato (Solanum tuberosum). Modalities and influence of phytohormones
1993
Penot, M. | Hourmant, A. | Reddahi, A. (University of Bretagne Occidentale, Brest (France). Plant Physiology Laboratory)
Sugar uptake by potato tuber discs was studied. Discs were used fresh or after a 24-h ageing period. It was shown that ageing increases (by 3 to 4 times) the rate of glucose and sucrose uptake. Sucrose uptake by fresh tissues was insensitive to the presence of glucose or fructose while a competitive effect was observed after ageing. This indicates the development of an invertase activity, which was inhibited by tris-buffer. Sucrose and glucose uptake by aged discs was dependent on cellular metabolism as shown by the sensitivity to low temperature and metabolic inhibitors (sodium cyanide, dinitrophenol, carbonyl cyanide m-chlorophenyl hydrazone). Involvement of thiol groups was demonstrated by the inhibition with NEM and PCMBS. Orthovanadate which decreases phosphate uptake by 85 % did not produce any effect on glucose and sucrose uptake by aged tissues. Fusicoccin produced only a slight stimulation (15 %). These results argue in favour of the involvement of specific adenosine triphosphatases (ATP) in ion and sugar uptake. No involvement of a redox system was observed. Abscisic acid (ABA) and benzylamino purine inhibited the uptake induced by ageing but had no effect on the endogenous sugar content. Benzylamino purine would act by its effect on the amount of ATP while ABA would act at the membrane level. The results are discussed in relation to the mechanisms of transfer of glucosyl groups and to the transport of sucrose by the symplasmic pathway.
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