Effect of aluminium and low pH on the callus induction and green plant regeneration in anther culture
1993
Kovacs, G. | Karsai, I. | Bedo, Z. | Barnabas, B. (Magyar Tud. Akad., Martonvasar (Hungary). Mezogazd. Kut.)
Due to the acidifiation of the soils the issue of aluminium toxicity will be more and more emphasized. A part of the varieties grown in Hungary possesses, however, a certain degree of tolerance, but it deemes to be advisable to increase this tolerance and to widen the group of tolerant genotypes. This may be achieved in the simpliest way by applying haploid mutant selection methods. But in order to develop an effective method, it is indispensable to know how the aluminium and low pH given to the induction nutrient medium would influence the anther induction and the fertile green plant regeneration. Based on the authors' results, the aluminium toxicity can be well simulated also in anther culture, the 5 micro M1 solution of Al3+ applied to the nutrient medium with low pH (pH: 4.2 and 4.5) reduces considerably the callus induction. The higher concentrations of AL3+ destroy entirely the microspore population. On regeneralion nutrient medium 190-2 with normal pH, the calluses originating from induction nutrient medium containing aluminium may be regenerated by adequate frequency. According to the seedling test results of grains originating from fertile dihaploid plants, an increase may be expected in the variability of aluminium tolerance even if the aluminium treatment is applied only in the induction phase. The multiplication of progenies and the testing of their aluminium tolerance are now going on
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