In vitro propagation techniques of tea [Camellia sinensis] plants
1991
Nakamura, Y. (Shizuoka-ken. Tea Experiment Station, Kikugawa (Japan))
Effective methods, simple and reliable, of in vitro propagation of tea plants have successfully been established. The growth of shoots in culture of shoot tips and axillary buds was remarkably accelerated when the media were mixed with the combination of BA(0.1-1.0 mg/liter)+GA3(5.0 mg/liter) or BA(1.0, 5.0 mg/liter)+(GA3(1.0 mg/liter). And it was observed that the axillary bud culture provided a more effective method, which was easier, simpler and quicker in securing the growth of shoots, as compared with the shoot tip culture. Differentiation of organs was observed on adventitious embryos (cotyledons used as the explants) and adventitious buds (stem segments used as the explants) cultured on the media containing BA(1.0-5.0 mg/liter) and IAA(0.01-1.0 mg/liter)+(GA3(1.0-5.0 mg/liter), respectively. A small shoot each developed from these differentiated adventitious embryos and buds on the same media. As the number of shoots grown was limited, passage culture by in vitro cutting of nodal segment culture with axillary buds was repeated to propagate them, for which the optimum combinations of hormones were IBA(0.1 mg/liter)+(BA(1.0 mg/liter)+(GA3(5.0 mg/liter) or IBA(0.1 mg/liter)+(2iP(5.0 mg/liter)+(GA3(5.0 mg/liter). By repeating this technique of nodal segment culture every 2 months, at least 47,000 (i.e. 6 raise to sixth power) plantlets could be multiplied from an original plantlet in a year. And rooting from these shoots was easily induced on 1/2 or 1/4 MS media incorporated with IBA(0.5-1.0 mg/liter)
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