Rapid detection of cucumber mosaic virus by a simplified F(ab')2 ELISA using PEG
1991
Maeda, T. (Okayama Univ., Kurashiki (Japan). Research Inst. for Bioresources) | Inouye, N.
Direct and indirect procedures of double antibody sandwich ELISA with simultaneous incubation of sample and conjugate were compared for their suitability to detect cucumber mosaic virus in sap from infected plants. Although CMV could be reliably detected by both procedures, indirect F(ab')2 ELISA using protein A-conjugate gave lower background levels than the direct procedure. In F(ab')2 ELISA, including 2% PEG in the reaction mixtures resulted in acceleration of the reaction and in higher ELISA values for infected samples. An incubation time of 2 hr was sufficient for reliable detection of CMV in sap infected plants by the procedure described
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