A biosensor for rapid determination of flatulence causing oligosaccharides in pea
1995
Voldrich, M. | Marek, M. (Vysoka Skola Chemickotechnologicka, Prague (Czech Republic). Ustav Konzervace a Technologie Masa) | Kvasnicka, F. | Vrbova, E.
The biosensor based on a Clark-type oxygen sensor with co-immobilized galactose oxidase and catalase was prepared and use for the determination of total content of alfa-galactooligosaccharides (raffinose, stachyose, verbascose, ajugose) in peas. The optimal conditions of sample preparation and measurement as well as the properties of biosensor were determined. The galactose biosensor is known to be less specific than e.g. glucose oxidase sensors, therefore the results were compared with those obtained by HPLC. For results of 16 pea cultivars the good correlation between total content of flatulence causing oligosaccharides determined by biosensor and HPLC was found.
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