Efficient production of gene products by repeated fed-batch fermentation using multiple fermentors [using Escherichia coli harboring plasmid encoding the beta-galactosidase structural gene]
1994
Fujioka, K. (Kyushu Inst. of Technology, Kitakyushu, Fukuoka (Japan)) | Shimizu, K.
In the cultivation of genetically engineered microorganisms, cell growth decreases after induction, and it becomes eminent when a product such as beta-galactosidase is harvested, indicating the conventional repeated fed-batch operation cannot be used to increase productivity. In this study, the use of multiple fermentors to overcome such difficulties was attempted. A mathematical model was developed based on fed-batch experiment using Escherichia coli JM103 harboring the pUR2921 plasmid encoding the beta-galactosidase structural gene. Computer simulation demonstrated that improved performance can be attained by repeated fed-batch operation using multiple fermentors. Experiments were then conducted to ascertain the operating conditions
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