Tracing effects of Na+ on morphology, phosphate metabolism and accumulated compounds of a sugar beet [Beta vulgaris] root by NMR
1993
Kano, H. (National Inst. of Agrobiological Resources, Tsukuba, Ibaraki (Japan)) | Ishida, N. | Takagishi, H. | Shirata, K. | Koizumi, M.
Effects of Na+ on morphology,phosphate metabolism and accumulated compounds of sugar beet root were studied by 1H-NMR imaging, and 31P-, 23N-, 23Na- and 13C-NMR. 23N-NMR showed that Na+ concentration of the sugar beet root reached 9.2 mM in the medium containing 180 mM NaCl. Broadening of 31P-NMR signal of vacuole-associated inorganic phosphate upon addition of Na+ suggests that the sugar beet root cells are furnished with the mechanism that alleviates the injurious effect of Na+. Strong water signal was observed in epidermal and vicinal cells, and around vascular bundles in 1H-NMR images, which was reduced by Na+ treatment. Based on 1H-NMR images and pictures of tetranitro blue tetrazolium (TNBT) staining, water was located in tissues with metabolically active cells. 13C-NMR spectra of 80% ethanol extracts of sugar beet roots showed accumulation of sucrose, betaine and glutamine. Extra accumulation of sucrose and betaine in parenchyma cells by Na+ treatment resulted in the reduction of active cells. Since Na+ treatment suppressed enlargement of sugar beet roots, the plant did not acquire resistance to grow against strong salt stress by long-term acclimation, though the results of this investigation showed that the plant is, by nature, tolerant to salt stress
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