Tissue-dependent expression of the rice wx(+) gene promoter in transgenic rice [Oryza sativa] and petunia [Petunia hybrida]
1995
Hirano, H.Y. (National Inst. of Genetics, Mishima, Shizuoka (Japan)) | Tabayashi, N. | Matsumura, T. | Tanida, M. | Komeda, Y. | Sano, Y.
The waxy (wx) locus, which controls the amylose synthesis, is known to be expressed specifically in the endosperm and pollen. To study the tissue-specific regulation of the wx+ gene, we introduced fusion gene that consisted of the upstream sequence of the Wx+ gene and the gene for beta-glucuronidase (GUS) into cells of rice (Oryza sativa L.) and petunia (Petunia hybrida L.). GUS activity was examined in the regenerated transgenic rice and petunia plants. In transgenic rice, the upstream sequence of the wx+ gene was sufficient to direct the tissue-specific expression of GUS in the endosperm and pollen, and the control of expression was quantitative. By contrast, in transgenic petunia, the same fusion gene was expressed in pollen but not in the endosperm. These results suggest that the putative cis-acting elements that direct pollen-specific expression are common to or similar in both monocotyledonous and dicotyledonous plants, whereas cis-elements responsible for the endosperm-specific expression of the rice wx+ gene do not function in petunia in which development of the endosperm differs from that in rice
显示更多 [+] 显示较少 [-]