Diagnostic methods of minks for providing with main vitamins
1995
Kvartnikova, E.G. | Kordyukov, N.P.
In order to develop methods of determining vitamins the most essential for mink activity the content of A1, B1, B2, B6, C and E vitamins and their metabolites in blood plasma was studied under different vitamin consumption rate. The content of retinol in blood and livers of animals became higher with introduction of excessive quality of vitamin A in mink ration. Decreased daily dose of vitamin E more than 6 times in the ration of animals against the standard one caused lower content of tocopherol in blood 2,8 times. Concentration of vitamin C in blood considerably increased with additional high dose (30 mg) in diet. Tenfold increase of tiamin in mink ration caused full vitamin B1 saturation. Providing of minks with vitamins was determined by activity of ferments in hemolysate of blood erythrocytes. Growth rate of transketolase activity and degree of FAD-effect with regard to saturation with vitamin B2 was demonstrated. Excessive vitamin B6 in mink diet caused lower degree of PALF-effect and on contrary this figure became larger under shortage of pyridoxine as pyridoxalphosphate. High reliability of methods proposed for identification of vitamins in mink blood was proved by complex computer analysis
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