Effect of phytohormones on biomass assumption and contents of ginsenosides in callus cultures Panax ginseng C.A. Mey
1995
Konstantinova, N.A. | Makhan'kov, V.V. | Uvarova, N.I. | Samoshina, N.F. | Sova, V.V. | Mikhajlova, O.M.
Callus strains of ginseng R-1 and 1c have been investigated with respect to the action of phytohormones on their growth and accumulation of ginsenosides. It was established that phenoxyacetic acid derivatives, analogous to auxine in physiological action, provide efficient growth of ginseng callus tissue and exceed in this characteristics indolyl acetic acid, alpha- and beta-naphtyl acetic acids and also indolyl butiric acid. Increase of the number of chlorine atoms in the molecule of phenoxyacetic acid from one to three leeds to decrease of the contents of ginsenosides in the culture R-1. The best hormone to provide the effect growth and biosynthesis of ginsenosides is 4-chlorine phenoxyacetic acid (4-CPA). While culturing on the medium containing this hormone callusses appear to be protrofic with respect to cytokinin. Kinetin and isopentenyl adenine (2iP) inhibite the growth of ginseng callusses in the presence of 4-CPA (strain 1c) and reduce the amount of ginsenosides. Inhibiting of the callus growth by zeatin is less significant. This hormone stimulates the ginsenosides biosynthesis at high concentrations of 25 and 75 mg/l that differentiates it from kinetin and 2iP
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