Identification of root knot nematode-induced genes in Arabidopsis thaliana
1994
Karimi, M. | Barthels, N | Montagu, M. van | Gheysen, G. (Gent Univ. (Belgium). Faculty of Agricultural and Applied Biological Sciences. Lab. for Genetics)
Root knot nematodes are the most destructive pest among plant-parasitic nematodes with nearly a world-wide distribution. So far, root knot nematode infection is controlled by crop rotation, pesticides, biological control, or by using resistant cultivars. Recently, molecular analysis of nematode/plant interactions have been started. Results from these studies can be used for genetic engineering of root knot nematode resistance in plants. One approach is to identify genes that are induced by nematode infection by using a promoter-tagging strategy. Therefore, an Agrobacterium tumefaciens strain harboring a beta-glucuronidase (gus) fusion vector has been used for transformation of Arabidopsis thaliana. Regenerated transformants contain the T-DNA inserted into different plant loci. The promoterless gus gene can be activated by a flanking plant promoter. Activation of the reporter gene upon nematode infection indicates that a nematode-inducible gene is tagged. We have already regenerated a thousand transgenic Arabidopsis plants and a part of them has been screened for gus expression after root knot nematode infection.
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