Laparoscopic transfer of fresh porcine embryos
1997
Riha, J. (Vyzkumny Ustav pro Chov Skotu, Rapotin (Czech Republic)) | Cunat, L. | Cerovsky, J. | Vejnar, J. | Horky, F.
Laparoscopic transfer of fresh porcine embryos was executed in three groups. Nulliparous gilts of the Czech Expressly Meat breed were used as recipients. Sows-donors of the Large White (LW) breed were applied the identically different dose of PMSG. Laparotomy was used for embryo recovery, in the dorsal position in linea alba after general sedation and anesthesia, following 24-hour fasting in the conditions of practical operations. The recipients were administered Regumate, application of PMSG and HCG followed in about 24 hours later. On average 15.5 embryos in the stage of morula to early blastocyst were transferred laparoscopically by three or four punctures to recipients of the 1st group; two out of four recipients conceived which delivered 14 piglets out of the total of 62 transferred embryos (22.6 %): among them 13 piglets were live, 12 piglets were weaned. A total of 141 embryos in the stage of 8 to 16 cells were transferred laparoscopically to eleven recipients of the 2nd group after the same treatment as in recipients of the 1st group, but one day earlier in the sexual cycle. Two recipients conceived, but they died during their pregnancy. Transferable embryos were transferred in the stage of 8 to 16 blastomeres to 10 recipients in the 3rd group, one of them conceived (10 %) and delivered 7 piglets that were weaned, which corresponds to 54.69 % (7/12.8) as the average number of transferred embryos per recipient and to 6.09 % as the total number of transferable embryos (7/115) in the 3rd group.
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