Fertility and morphology of the chromosome-doubled hybrid Trifolium medium x T. pratense (red clover) and backcross progeny
1995
Sawai, A. (Hokkaido National Agricultural Experiment Station, Sapporo (Japan)) | Yamaguchi, H. | Uchiyama, K.
In order to transfer rhizomatous growth habit of T. medium (2n=80) into T. pratense (2n=28), a chromosome-doubled interspecific hybrid was produced by colchicine treatment of hybrid embryos. Pollen fertility of the hybrid, the first and the second backcross progeny of the hybrid to T. pratense, were 1.1%, 0-4.6%, and 2.3-36.8%, respectively. The second backcross progeny produced normal seeds when pollinated with T. pratense. Chromosome number of the third backcross progeny ranged from 2N=32 to 42. Backcross progeny produced rhizomes, which should allow long-term persistence of the stands. Backcross progeny with acceptable levels of fertility produced in this study provides useful material for the development of persistent T. pratense cultivars. Recurrent selection of T. medium for interspecific compatibility would further increase fertility of chromosome-doubled hybrids and backcross progeny
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