Evaluation of radioreceptor assays for avian gonadotropins
1995
Iwasawa, A. (Gifu Univ. (Japan). Faculty of Agriculture) | Hoshino, M. | Fukuhara, Y. | Doi, O. | Kawashima, M. | Kamiyoshi, M. | Nakamura, T.
Four different kinds of radioreceptor assays wee performed to measure avian gonadotropins. For lutropin assays, ovaries of immature female rats treated with pregnant mare serum gonadotropin (PMSG) followed by human chorionic gonadotropin, or testes of mature male rats were PMSG, or testes of mature male Japanese quails were used. Rat lutropin and follitropin were used for reference preparations of the assays and for labeling with iodine-125. Pituitary glands of laying hens were homogenized with distilled water. When the homogenates were serially diluted and assayed by the four different systems, they cross-reacted to the two lutropin assays (though with low sensitivity) and to the follitropin assay with rat ovaries, but not at all to the follitropin assay with quail testes. Further characterization should be required for these assays to be used for accurate and hormone-specific measurement of chicken gonadotropins
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