[Gene mapping in almond trees using RAPD markers]
1996
Joobeur, T.
法语. This study has used an F1 progeny of a cross between two almond cultivars ('Ferragnes' and 'Tuono') to include RAPD markers in a genomic map of almond (Prunus amygdalus Batsch) constructed with the same population by Viruel et al. (1995) using RFLP and isozyme markers. Then it has screened 325 primers of arbitrary sequence, and we selected 41 to amplify 56 RAPDs. Only RAPDs segregating as backcrosses (the band present in one parent and absent in the other) were chosen. These RAPDs were mapped with the mapping criteria used by Viruel et al. (1995), (LOD equal or greater than 3 and recombination frequency equal or less than 30 cM). Thirty-five were found in the maternal map ('Ferragnes' map) and 21 in the paternal map ('Tuono' map). All mapped RAPDs were found in those linkage groups identified by Viruel et al. (1995). The eight linkage groups identified cover 456 cM in the 'Ferragnes' map and 476 in the 'Tuono' map, with an increase of 16 and 21 per cent, respectively of previous map distances. Bulk segregation analysis (BSA) was used in two F1 progenies ('Garrigues' x 'Tuono' and 'Garrigues' x 'Genco') to target the kernel bitterness and the self-compatibility loci of almond with RAPD markers. Two markers flanking the bitterness locus were identified but none was found for the selfcompatibility gene. The efficiency of the BSA according to the population used (F1 progeny, F2 and BC) was discussed.
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