Genetic variation of a single pollen-derived doubled haploid population in rice
1996
Moon, H.P. | Kang, K.H. | Choi, S.H. | Ahn, S.N.
Somaclonal variation was observed in the field in doubled haploid plants derived from pollen culture of the anther-derived japonica variety Hwaseongbyeo. The variations in seven quantitative traits including plant height and one qualitative trait (pubescence) in 436 lines (R2 generation) were analyzed. R2 lines that fell outside the 95% confidence interval of the original variety were checked for each trait. The variations in quantitative traits, except days to flowering, appeared to be inferior to Hwaseongbyeo. Variability within the same R2 line was also seen for quantitative and qualitative traits. Twenty-nine R2 lines, segregated for pubescence and 130 segregated for fertility, suggesting that mutations usually occur before diploidization. Twenty-nine R2 lines, representing a wide spectrum of variation, were chosen for random amplified polymorphic DNA analysis. The number of lines showing DNA polymorphisms compared with the original variety ranged from 0 to 10, depending on the primer used. This seems to indicate that some primers can span a highly mutable genomic site
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