Preliminary survey on proto-oncogenes in normal Thai domestic animals
1990
Ted Tesprateep | Boonmee Sunyasootcharee | Achariya Sailasuta (Chulalongkorn Univ., Bangkok (Thailand). Faculty of Veterinary Science. Dept. of Veterinary Pathology)
Information about oncogenes in domestic animals have been previously attempted. This present study, we investigated the protooncogenes homologous to the c-myc, c-ros-1, c-yes-1 and v-K1-ras oncogenes of genomic DNAs in normal Thai domestic animals, including buffalo, dog, cat and duck with Southern blot hybridization. High-molecular-weight-genomic DNAs in each of the animals contained proto-oncogenes showing more than 65 % homology of the proto-oncogenes detected band differed band differed in both number and approximate molecular weight: i.e. one or two bands at 1.6 to 18.0 kilobase (kb) in the c-myc probe, one band at 1.1 to 15.0 kb in the c-ros-1 probe, one to five bands at 0.3 to 18.0 kb in the c-yes-1 probe and one to five bands at 1.6 to 18.0 kb in the v-Ki-ras probe. The degree of auto-radiographic signal strength of each band among these animals was inconsistant for each probe. The results obtained should provide some data for molecular biological studies of tumors in domestic animal in Thailand.
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