Electron microscopic detection of feline leukemia virus in cats
1990
Parnchitt Nilkumhang (Kasetsart Univ., Bangkok (Thailand). Faculty of Veterinary Medicine. Dept. of Veterinary Medicine) | Maliwan Choontanom | Nisit Muangsri
Serum from cats with non-specific illness were tested for the presence of feline leukemia virus (FeLV) antigen and feline immunodeficiency virus (FIV) antibodies utilizing enzyme-linked immunoassays. The four selected cats with positive feline leukemia virus infection were sacrificed for electron microscopic study. Spleen, bone marrow and lymph nodes were examined for the presence of virus particles. Among several retroviruses of domestic cats, feline leukemia virus (FeLV) is the most important virus pathogen causing a wide variety of disease syndromes including hematopietic tumors, anemias and various acquired immunodeficiency disorders. In Thailand the epidemiologic survey of FeLV infection was firstly conducted in 1987 employing the ELISA test in which 0.9 % of stray cats and 20.9 % of illed cats were positive. Furthermore, the observation resulted that the most frequent FeLV associated diseases were chronic glomerulonephritis and anemias. Several workers revealed the presence of FeLV in tissue of infected cats by electron microscopic study either directly or indirectly from inoculated tissue culture. The C-type virus particles of FeLV are morphologically similar to murine and avian leukemia viruses, of approximately 110 mu and are mostly demonstrated from spleen, bone marrow, lymph nodes and blood platelets.
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