Enhanced efficiency micropropagation of mangosteen through young leaf culture
1991
Sompong Te-chato | Mongkon saelim | Arunee Muangkaewngam (Prince of Songkhla Univ., Songkhla (Thailand). Faculty of Natural Resources. Dept. of Plant Science)
Direct embryogenesis was obtained from culturing young leaves of mangosteen (Garcinia mangostana L.) in liquid medium. The medium was MS which was reduced concentration of the component to half of origin and supplemented with low concentration of auxin and cytokinin. The cultures were maintained under 3,000 lux illumination for 16 hour photoperiod. After culturing for 4 weeks, multi-shoot buds were induced with 33.33 percent. A number of shoot buds obtained from these cultures were recorded to be 40.37 buds. A large number of shoot buds were crowded at both rims/edges near leaf apex and scatter to the midrib. A comparative result for shoot bud formation between seed and leaf culture in various media and phytohormones showed that culture of young leaves in liquid medium could be amended to yield a great percent and number of shoot buds as same as culture seed. Histological studies of leaf-derived multi-buds revealed that the buds come out from two sources, one from vascular cambium and another from epidermal cell.
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