Genetic relationships among commercial corn hybrids and parents based on RAPD analysis of pericarp and embryo DNA
1996
Stojsin, D. (University of Guelph, Guelph (Canada). Department of Crop Science) | Kannenberg, L.W. | Rajnpreht, J. | Pauls, P.K. | Stojsin, R.
In this paper, contrary to theoretical expectations, the results obtained indicated that the RAPD (Random Amplified Polymorphic DNA) patterns obtained from hybrid pericarp DNA were not the same as those obtained from maternal inbred line embryo DNA. However, they were comparable to the markers obtained from the pericarp DNA of the maternal inbred line. Furthermore, the hybrid pericarp data generally agreed with the parental pedigree information available to us. Thus, hybrid pericarp DNA can be used for maternal inbred line identification. Hybrid pericarp DNA data, complemented by hybrid embryo DNA results, can be used in diversity studies as an indicator of relationships among hybrids and their parental inbred lines. The study also showed that diversity among commercial corn hybrids grown in Canada is limited and that similar inbred lines are frequently used as parents in more than one hybrid.
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