Comparison of the phagocytic function of sheep alveolar macrophages harvested from healthy or pneumonic lungs | Evaluation, in vitro, de l'activite phagocytaire de macrophages alveolaires provenant de moutons sains ou atteints de pneumonie
1997
Belayat, F. | Dewaele, A. (Universite de Liege, Sart Tilman (Belgium). Faculte de medecine veterinaire. Service de pathologie generale) | Meniai, K. | Coignoul, F.
The lesions of pulmonary parenchyma in ovine species appear to evolve rapidly in spite of the fact that many defense mechanisms exist in the lung. The purpose of this study was to evaluate the phagocytic capacity of alveolar macrophages issued from pneumonic sheeps compared to phagocytic activity of alveolar marcophages issued from healthy sheep. Pulmonary macrophages were collected by broncoalveolar lavage and separated on Percoll density gradient (Pharmacia). Phagocytic activity was evaluated by using fluoresceine labelled and opsonized yeast particles. After incubation, the phagocytic cells were counted and the number of ingested particles per cell was determined. The results showed a significant difference between the number of phagocytic alveolar macrophages between healthy and pneumonic sheep (96 per cent and 77 per cent respectively). In contrast, between the alveolar macrophages of the both healthy and pneumonic lungs, there is no significant difference in the number of ingested particles per cell
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