Effect of Azotobacter strains on sugar beet callus proliferation and nitrogen metabolism enzymes
1998
Mezei, S. | Kovacev, L. | Mrkovacki, N. | Nagl, N. (Institute of Field and Vegetable Crops, Novi Sad (Yugoslavia)) | Popovic, M. | Malencic, D.
The effect of five Azotobacter chroococcum strains and nitrogen content in nutrient media on callus growth of two Beta vulgaris cultivars were investigated, as well as the activity of nitrate reductase (NR), glutamine synthetase (GS) and glutamate dehydrogenase (GDH) in inoculated callus tissue. On medium with full nitrogen content (1 N) the inoculation with A. chroococcum strain A2 resulted in the highest calli mass, while strains A8 and A14 maximally increased NR activity. On media with 1/8 N the highest effect on calli growth, GS and GDH activity had the strain Ag. The strain A2/1 significantly increased callus proliferation on medium without N. Asymbiotic association between sugar beet calli and Azotobacter depended on genotype/strain interaction and was realized in presence of different nitrogen levels.
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