Assessment of general toxicity of plant pollutants by means of bioassay
1997
Pavel, Ue. (Estonian Agricultural Univ., Tartu (Estonia))
It is recommended that the embryotoxicity and immunosuppressive effect of pollutants be assessed during the perinatal period of chick development (measuring the perinatal mortality). On the 9th day of incubation the chloroform-ethanol (80:20 v/v) extracted material suspension in plant oil was injected into allantois (0.1 ml), as in Adebajo and Oyesiku (1994). The embryonic mortality is registered on the 12th and 18th incubation day and on the day of hatching. For the assessment of the immunosuppressive impact of the plant extract we are guided by R.R. Lochmiller et al. (1993). On the 10th post-hatching day the New Hampshire chicks (20 fertilized eggs were taken as control and 20 for the experiment) were challenged subcutaneously with Salmonella gallinarum killed cells (0.1 ml, 10**8 cells). On the 8th postvaccinational day the chicks were bled and the specific agglutinin titers (log2) were determined. The body mass, the mass of the spleen and bursa Fabricius were also determined. On the 15th day of their life, the CMI of the chicks were assessed by a phytohemagglutinin (PHA-P, Sigma, USA) skin test, injecting 0.05 ml (100 micro grams). After 24 hours the reaction was determined
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