Isolated microspore culture in wheat (Triticum aestivum L.): the effect of co-culture of wheat or barley ovaries on embryogenesis
1996
Bruins, M.B. (DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), Wageningen (Netherlands)) | Rakoczy-Trojanowska, M. (Warsaw Agricultural University, Warsaw (Poland). Department of Horticultural Plant Genetics and Breeding) | Snijders, C.H. (DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), Wageningen (Netherlands))
In this study the effects of different ovary co-culture methods on sporophytic development of wheat microspores were investigated. Mechanically isolated microspores of greenhouse grown donor plants of wheat (Triticum aestivum L.) were co-cultured with wheat or barley ovaries either in agarose rings or in culture plate inserts. The viability of the microspores was better in co-culture with wheat ovaries from one cultivar as compared to co-culture with a mixture of wheat ovaries from four cultivars. Co-culture of the microspores with ovaries in culture plate inserts had no significant effect on viability of the microspores, but had, in comparison with culture in agarose rings, a large positive effect on the percentage swollen microspores. No clear genotype effect could be detected. A significant correlation between the number of swollen microspores and the number of multicellular structures was found
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