In-vitro culture induced deficiency in barley
1997
Hang, A. | Satterfield, K. (USDA/ARS and University of Idaho, Aberdeen, ID (USA))
A plant with a structural chromosome deficiency was isolated from a population of 407 green plants regenerated from microspore culture of ten barley (Hordeum vulgare L.) cultivars. Based acetocarmine staining and N-banding patterns, the deficient chromosome was identified as chromosome 1 with 67 percent of the long arm missing, acrocentric chromosome 1SL. At meiosis, six ring bivalents and one open bivalent were observed. Among 111 progenies that resulted from selfing of the deficient plant and crossing with a diploid, all had 14 chromosomes. This indicated that the deficient chromosome was not transmitted through male or female gametes. The deficient plant was late and had reduced vigor and seed set
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