Use of pollen storage and detached-tiller culture in wheat polyhaploid production through wide crosses
1997
Inagaki, M.N. (International Maize and Wheat Improvement Center (CIMMYT), Mexico (Mexico)) | Nagamine, T. (National Agriculture Center (NARC), Tsukuba, Ibaraki (Japan)) | Mujeeb-Kazi, A. (International Maize and Wheat Improvement Center (CIMMYT), Mexico (Mexico))
The effects of pollen storage and detached-tiller culture on polyhaploid production of hexaploid wheat were examined using maize and pearl millet crosses. Pollen storage at ultra-low temperature did not affect polyhaploid production frequency in pearl millet crosses, but greatly reduced frequency in maize crosses. Stored pearl millet pollen can be used as an alternative medium for wheat polyhaploid production when fresh pollen is not available. Detaching tillers with spikes at crossing time and culturing them in a solution containing sucrose, sulfurous acid and 2,4-dichlorphenoxyacetic acid (2,4-D) resulted in no distinct of polyhaploid production frequency in crosses with both maize and pearl millet. Hot-water emasculation after detaching wheat tillers was successful for these wide crosses. Detached-tiller culture makes it possible to collect the spikes from wheat plants growing in distant sites and handle them for wide crosses in a laboratory. These techniques avoid having to synchronize flowering times of both parents and result in considerable savings in terms of labor and space required for handling parent plants
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