HPLC analysis of vitamin B6 derivatives in the silkworm, Bombyx mori
1997
Huang, L. (Gifu Univ. (Japan)) | Zhang, J. | Hayakawa, T. | Tsuge, H.
The existence of vitamin B6 compounds in silkworm organs was studied with high-performance liquid chromatography (HPLC). The pyridoxine (PN) content in the organs of silkworms raised on mulberry and artificial feed was significantly higher than the coenzyme forms, pyridoxal 5'-phosphate (PLP) and pyridoxamine 5'-phosphate (PMP). In the 5th instar and pupae, changes in PN content in various organs was observed. It was thus thought that PN in mulberry and artificial feed was at first taken up easily in the cell of midgut, then moved to the hemolymph. At the early 5th instar, the PN mainly was collected and stocked in the fat body and the malpighian tube, and at the later 5th instar, almost all PN was found in the silkgland. For metabolism of vitamin B6 in the silkworm, the silkworm may act as an excrete organ for excess PN in the body. After cocoon formation, nearly all of the PN in various organs was moved to the fat body, then into the hemolymph, and it was finally taken up in the ovaries. Surprisingly, the PN content in silkworm eggs reached approximately 1,920 nmol/g. The contents of coenzyme forms of vitamin B6 (PLP and PMP) increased with the enhanced biosynthesis of the silk protein in the posterior silkgland and the fat body
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