Comparative changes of lipogenic-related enzyme activities by dietary glycerol tricaprylate, tricaprate, trilaurate and trioleate in rat liver and adipose tissues
1996
Torii, S. (Kyoto Univ. (Japan). Coll. of Agriculture) | Hwang, S.G. | Matsui, T. | Yano, H.
The present study was undertaken to investigate the effects of the carbon chain length of dietary medium chain triacylglycerols on lipogenic-related enzyme activities in growing rats. Twenty-four Wistar strain male rats (8 weeks old) were allotted to 4 groups and fed the diets containing glycerol tri-homogeneous fatty acid (260 g/kg diet) composed of only caprylic acid (C8:0), capric acid (C10:0), lauric acid (C12:0) or oleic acid (C18:1). After four weeks, the activities of three lipogenic-related enzymes (glucose-6-phosphate dehydrogenase, G-6-PDH; citrate cleavage enzyme, CCE; and malic enzyme, ME) in liver and two adipose depots (perirenal and epididymal) were measured. Feeding of C8:0 diet resulted in significantly higher G-6-PDH and CCE activities in the liver than C10:0 feeding. In perirenal adipose tissue, G-6-PDH and ME activities were higher in C8:0 group than those in the other three groups. The activity of CCE in this adipose depot was lower in C8:0 group than in C10:0 and C12:0 groups. In epididymal adipose tissue, activities of G-6-PDH and ME were lower in C8:0 group than in C12:0 group. Fatty acid composition of perirenal adipose tissue demonstrated that significant amount of dietary C10:0 and C12:0 fatty acids was directly incorporated into9 adipose tissue, whereas C8:0 deposition was too small to be detected. These results suggest that dietary C8:0, C10:0 and C12:0 fatty acids differently affect lipogenic-related enzyme activity in both liver and adipose tissues in rats
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