In vitro shoot and root proliferation in flowering mume [Prunus japonica] cultivars
1996
Murai, Y. (Shizuoka Univ. (Japan). Faculty of Agriculture) | Harada, H. | Takagi, T.
Optimal micropropagational conditions for shoot proliferation and rooting in vitro were investigated using 3 flowering mume cultivars, Ichinotani, Hakubotan and Yae-bungo. The effects of three types of cytokinin and their concentrations on shoot proliferation were tested with Ichinotani. (1) Benzyladenine (BA) at 5 or 10 mu-M was most effective for shoot proliferation, whereas zeatin (Zn) was not, however, BA and Zn were both effective for shoot elongation. N-(2-chloro-4-pyridyl)-N-phenylurea (CPPU) had little effect on shoot proliferation and shoot elongation. (2) The use of sorbitol, sucrose and glucose on survival rates, shoot proliferation and shoot elongation on the three cultivars revealed that the survival rate was higher on the medium containing sorbitol than on the medium containing sucrose or glucose. Sorbitol was also most effective for shoot proliferation, whereas glucose was most promotive for shoot elongation. Sucrose had little effect on shoot proliferation and shoot elongation. (3) IBA treatments enhanced rooting. Except for Ichinotani, the number of roots and rooting percentages were higher as IBA concentration increased. For Ichinotani, optimal IBA concentration was 1 mu-M. Dark treatment for 10 days in combination with 1 mu-M IBA gave higher rooting percentages and number of roots than those receiving IBA treatment alone
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