Content and characteristics of vitamin B12 in some seaweeds
1996
Yamada, S. (Hokkaido Univ., Sapporo (Japan)) | Shibata, Y. | Takayama, M. | Narita, Y. | Sugawara, K. | Fukuda, M.
The vitamin B12 (B12) content in seven species of seaweed that are consumed frequently in Hokkaido, Japan, was microbiologically measured using Escherichia coli 215. Asakusanori (Porphyra tenera), maruba-amanoir (Porphyra suborbiculata) and akaba-gin-nansou (Rhodoglossum pulcherum) showed higher B12 content than the other species, although the content varied greatly among samples in the same species. A bioautography on the thin-layer plate holding a mixture of silica gel and cellulose, differentiation of B12 and its analogues using a binding specificity of intrinsic factor and haptocorrin, and comparison of the B12 concentration determined by the radioisotope dilution assay method using the intrinsic factor as the B12-binding protein with that by the bioassay method, predominantly showed B12 in maruba-amanoir and B12 analogues in wakame (Undaria pinnatifida) and akaba-gin-nansou. The B122 uptake of akaba-gin-nansou fro artificial seawater was similar to that of asakusanori that contained only B12
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