Biological control of mango stem-end rot caused by Diplodia natalensis pole Evans with Trichoderma viride Pers.
1995
Moreno, L.S. | Paningbatan, R.A. (Visayas State Coll. of Agriculture, Baybay, Leyte (Philippines). Dept. of Plant Protection)
Trichoderma viride consistently inhibited mycelial growth and suppressed the sporulation capacity of Diplodia natalensis in agar culture by 99 percent. In vivo efficacy test of T. viride at 10 to the eighth power conidia/ml resulted in significant reduction in disease incidence from 100 to 20 percent translating into 80 percent level of protection of mango fruits. Treatment of T. viride at 10 to the eighth power conidia/ml provided 67.9 and 63.4 percent biocontrol efficacy based on diseased fruit surface area and fruit volume rotten, respectively. Such efficacy levels were comparable to that provided by benomyl. T. viride completely protected mango fruits from being infected when inoculum levels of D. natalensis was 15,000 conidia/ml or less. The levels of biocontrol declined from 95-35 percent when pathogen inoculum increased from 20,000-50,000 conidia/ml. Simultaneous application of T. viride and of D. natalensis caused the lowest diseased incidence and severity of stem-end rot. Delaying T. viride application after inoculation of D. natalensis resulted in lower disease control than before inoculation of the pathogen. T. viride alone did not cause any observable lesion on treated mango fruits
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