Effect of genotype and media composition on embryoid induction and plant regeneration from anther culture in triticale
1997
Slusarkiewicz-Jarzina, A. | Ponitka, A. (Polish Academy of Sciences, Poznan (Poland). Inst. of Plant Genetics)
Anthers of twenty triticale genotypes were cultured on three different media. The anthers were incubated in darkness at 30 deg C. After 3-4 weeks, the somatic embryos developed from microspores were transferred to a regeneration medium and illuminated for 16 h/day. Green plantlets were then transferes into tubes containing the same regeneration medium and subsequently transfered into pots. After vernalization green haploids were placed in a solution of colchicine (with additives) and kept for 12 h in continuous light at 22 deg C. Excess colochicine was removed and the plants were finally transplanted to pots. The chromosome number of the plants developed in vitro was determined in root-tip aquashes with acetocarmine
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