Biological screening and analysis of transgenic rice lines expressing coat protein gene(s) of rice tungro spherical virus
1996
Sivamani, E. | Shen, P. | Ong, C.A. | Hamidah, G. | Hassan, G. | Mohamed Senawi, M.T. | Beachy, R.N. | Fauquet, C.M.
For a growing number of positive-strand RNA viruses, it has been demonstrated that transformation of host plants with the viral coat protein gene confers resistance to the corresponding virus. In a similar approach, we have attempted to demonstrate coat protein-mediated resistance to rice tungro spherical virus. Earlier, we reported the successful transformation of rice varieties with three-coat protein gene cassettes. The offspring of the stably transformed rice lines were inoculated with the viruliferous green leafhoppers in greenhouse trials. Among many lines tested by double antibody sandwich-enzyme-linked immunosorbent assay and Northern blot analysis, a representative example, which has each of the coat protein genes and which showed significant disease resistance, is reported here
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