Genetic transformation of rice with viral genes for novel resistance to rice hoja blanca virus
1996
Lentini, Z. | Calvert, L. | Tabares, E. | Lozano, I. | Ramirez, B.C. | Roca, W.
Rice hoja blanca virus (RHBV) is present throughout much of tropical America. When epidemics occur, yield losses may reach 100%. Most Latin American varieties have the same resistance gene. This project aims to provide new sources of resistance to minimize the possibility of an outbreak of the disease. RHBV is a member of the tenuivirus group. Coat-protein cross protection and antisense-gene down regulation of the major nonstructural protein NS4 are being attempted. The antisense-gene strategy for the expression of RNA4 aims to determine the function of the major NS4 protein and to evaluate the potential for a different and complementary method ofproducing virus-resistant plants. Immature embryos or immature panicle-derived calli from three tropical irrigated Latin American indica varieties and two tropical upland japonica lines are used as targets. Direct gene transfer is performed using the PDS-1000/He system. Constructs containing the RHBV-CP or the RHBV-RNA4, and the 35S CaMV-hph gene are being tested. Previous molecular and inheritance analyses indicate that about half of the regenerants have stable integration of the hph gene. Preliminary Southern and Northern analyses show that some of the hph-resistant plants also contain and express one of the RHBV genes
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