Assessment of genetic diversity of Xanthomonas oryzae pv. oyzae in Nepal
1996
Adhikari, T. | Nelson, R.J. | Mew, T.W. | Leach, J.E.
Two DNA clones from Xanthomonas oryzae pv. Oryzae (Xoo), IS1112, an insertion sequence, and avrXa10, a member of a family of avirulence genes, were used as probes to analyze genomes of the 45 strains of Xoo, collected from different rice-growing ecosystems in Nepal. Thirty haplotypes were detected when the data from both pJEL101 and pBSavrXa10 were combined. Haplotype diversity was 0.98, suggesting high genetic variability within populations of Xoo in Nepal. Virulence of the randomly selected strains was determined by inoculating five differentials and 10 near-isogenic lines. Most of the strains were virulent to cultivars containing the bacterial blight resistance genes, while a majority of the strains was avirulent to Xa21
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