Effect of aluminium on root and leaf cytoplasmic proteins in maize
1997
Mistrik, I. | Tamas, L. | Huttova, J. (Slovak Academy of Sciences, Bratislava (Slovak Republic). Inst. of Botany)
Aluminium toxicity is believed to be one of the major factors limiting crop productivity in acid soils. Despite the complex nature of Al toxicosis, considerable evidence indicates that it is primarily associated with disruption of root physiology and function. In our study SDS-PAGE was used to monitor changes in gene expression following Al treatment (100 micro mol.l sup(-1) AlCl3). Cytoplasmic proteins isolated from root apex, root base and first leaf of maize seedlings were fractionated using anion exchange column Bio-Scale Q2. Two (root base) or three (root apex and first leaf) fractions of proteins were eluted from the column with linear 0-1.0 mol.l sup(-1) NaCl gradient. SDS-PAGE of cytoplasmic proteins isolated from maize seedlings treated with Al revealed marked differences in polypeptide compositions of cytoplasmic proteins isolated from root and leaf. Al stress enhanced accumulation of 8 polypeptides in the leaf cytoplasmic proteins fraction and 2 or 5 polypeptides in root cytoplasmic proteins, respectively. At the same time reduction of 9 polypeptide bands was observed in cytoplasmic proteins isolated from two different parts of maize root. Reduction of polypeptide bands in response to Al stress was not found in leaf proteins. The differences found in polypeptide compositions between root and leaf cytoplasmic proteins are the subject of on-going studies
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