Improvement of Lily Doi (Lilium primulinum Bak. var. Burmaniacum Stearn.) through embryo culture
1993
Kriangkrai Angumnuaysiri
Interspecific hybridizations among four divisions of lilies were made. Thai native lilies named Lily Doi (Lilium primulinum Bak. var. Burmaniacum Stearn.) were crossed to Longiflorum hybrids, Asiatic hybrids and Oriental hybrids. Hybridizations were unsuccessful when Lily Doi were used as male and the other divisions as female plants. On the other hand fruit set and seeds were developed when Lily Doi were used as female and the other divisions as male plants. Out of 177 cross pollinations, only 37 crosses were with fruit growth (20.90 percent). Average fruit age when harvested was 43.17 days. Average fruit weight was 1.23 gram. Average seed number per fruit was 105.66. Embryo rescue was made among these interspecific hybrids. Embryos were aseptic cultured in Murashige and Skoog media (MS). Germination percentage was 1.57 percent (66 germinated from total of 4,196). Only the germinated seeds were subcultured to MS media with addition of 0.1 milligram per liter of NAA and 60 gram per liter of sucrose. Fifty two embryos formed callus (78.78 percent) and further developed into 31 plantlets, 4 embryos developed directly to plantlets (6.06 percent) and 10 embryos were without growth (15.15 percent). Survival percentage of the first and second crops were 21.39 and 39.75 percent respectively.
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