Qualitative changes in the Kunitz trypsin inhibitor in soybean seeds during germination
1997
Zilic, S. | Bozovic, I. | Bekric, V. | Konstantinov, K. (Maize Research Institute "Zemun Polje", Belgrade - Zemun (Yugoslavia))
During soybean (Glycine max L. Merrill) seed germination, new forms of Kunitz soybean trypsin inhibitor (KSTI), genetically identical to the native forms of KSTI present in the dry seed, appear, but in contrast to the latter, they have a somewhat lower electrophoretic mobility. Using polyacrylamide gel electrophoresis, the new forms of KSTI were identified in two local soybean varieties (ZPS Bosa and ZPS 015) and an introduced one (Hodgson). Dry seeds of these varieties contain the Ti(a) genetic variant of KSTI. The new form Ti(a)m occurs on the third or fourth germination day depending on the genotype. In the course of time, the activity of this form increases, while the activity of the natural Ti(a) form decreases, but on the seventh-eight day from the beginning of germination both forms slowly disappear. New forms of KSTI could be the result of different gene expression of KSTI during germination and maturation or could arise after modification of native KSTI under the influence of proteases.
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