Development of chloroplast in the mesophyll cells of Satsuma Mandarin foliar sprayed with urea
1999
Aguja, S.E. | Mohammad, P. (United Graduate School of Agricultural Sciences, Matsuyama (Japan). Faculty of Agriculture)
The development of chloroplast in the mesophyll cells of control and urea-applied satsuma mandarin leaves were studied. At 20 days after budding (DAB) active differentiation of chloroplast in urea-applied trees was evident. However, on a length times width basis, chloroplast were small in both palisade (1.99 x 0.70 Mew m2) and spongy (2.78 x 1.50 Mew m2) layers that contained few small (0.53 - 0.72 x 0.39 - 0.57 Mew m2) starch grains. The thylakoid layers per chloroplast were lesser (2.80) in this treatment compared to control (3.72). At 40 DAB, both control and urea-applied trees reached chloroplast maturity, but control trees had bigger chloroplast (4.87 x 2.39 Mew m2) in the palisade layer cells which contained extremely large and numerous starch grains with few dilated thylakoid layers. Degenerating chloroplast in the spongy layer cells of control trees were characterized by the disappearance of the outer membrane. While urea-applied trees had smaller chloroplast (4.56 x 2.39 Mew m2) in the palisade layer. Further, few small starch grains, thylakoid-filled stroma and many lipid droplets were found. Moreover, thylakoid layers were about to dilate but outer membrane of the chloroplast remained intact in both palisade and spongy layer cells. Compared to the palisade layer cells, chloroplast in the spongy were bigger (5.32 x 1.95 Mew m2).
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