Genetic characterization at molecular level of Asian buffaloes (Genus bubalus) present in the Philippines
1998
Solis, C.D. (Philippines Univ. Los Banos, College, Laguna (Philippines). Dept. of Basic Veterinary Sciences)
Twenty blood protein loci from buffaloes present in the Philippines: eleven domestic buffaloes and seven tamaraws were examined electrophoretically to genetically characterize them at molecular level. Milk proteins from domestic buffaloes and cattle were also preliminarily studied to explore the possibilities of using milk proteins as genetic markers employing modified electrophoretic techniques. Based on blood protein electrophoresis, variations within the swamp buffalo, river buffalo and tamaraw were attributable to four polymorphic loci (Alb, Tf, DBP and CA-2). In addition, four loci (Hb alpha, ES, ALP and PGM), although not polymorphic within the three groups of buffaloes were found to be variable between the groups, differentiating the domestic buffaloes from the wild tamaraw. Genetic variability was higher in domestic buffaloes (H=0.076 and H=0.071 for the swamp and river types, respectively; both types had same Ppoly=0.150), while the tamaraw was low (H=0.025; Ppoly=0.050). The tamaraw displayed genetic uniqueness among the buffaloes examined, as well as among the Asian buffaloes. It was also apparent that the tamaraw is more genetically related to the swamp type than the river type of domestic buffalo. The examination of milk proteins revealed good resolution of bands, characteristic banding patterns and even variations in the milk proteins. Through these methods, buffaloes can be distinguished from cattle at molecular level. Milk protein polymorphism was also demonstrated within buffalo and/or cattle groups suggesting that certain milk protein loci can be useful genetic markers for these animals
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