Occurrence of cotton leaf curl virus (CLCuV) in Sindh [Pakistan]
1997
Khalid, S. (National Agricultural Research Centre, Islamabad (Pakistan). Crop Diseases Research Inst.) | Soomro, M.H. | Ahmad, I.
A program of monitoring virus diseases with emphasis on geminiviruses including CLCuV in Sindh was initiated in late 1996. Hyderabad, Sanghar and Nawabshah districts were surveyed during September, 1996 and the districts of Ghotki, Sukkur, Khairpur and Naushehro Feroze during January, 1997. CLCuV-like symptoms showing curling, cupping upward/downward and shortening of leaves, thickening of veins and development of enations of underside of leaves were observed in cotton fields around Ubaro in district Ghotki. The presence of CLCuV was confirmed through Triple Antibody Sandwich Enzyme Linked Immunosorbent Assay (TAS-ELISA) using panel of Monoclonal Antibodies against whitefly-transmitted geminiviruses (WTGs), polymerase chain reaction (PCR) using CLCuV- specific primers and by reproducing CLCuV symptoms in cotton (cv. S-12) through whitefly inoculation under controlled conditions.
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