Pathogenicity of Aeromonas hydrophila in Rana tigerina
1994
Theerawoot Lerssuthichawal | Wanna Nontanapan | Krissana Chuchuai (Rajamangala Institute of Technology. Faculty of Agriculture Nakhon Si Thammarat (Thailand))
Two size-groups of Rana tigerina were intra-muscularly injected with Aeromonas hydrophila suppension (5 * 10 ** (10) cells/individual). The symptoms of disease were appeared within 12 hours, with lethargy, loss of appatize, pale in color and edematous fluid between skin and muscle layer. Internal organs enlarged and changed in color. The septicemia was company developed within 18 hours. Mortalities were observed within 48 hours, 46.6 % of the large frogs and 36.6 % of the small one, respectively. Ulcerative lesion, red leg and infected internal organs were observed in the moribun frogs. The survivals were completely recovered within 6 days. The component of blood corpuscles was also studied. The number of red blood cells of all infected frogs were slightly decreased since 6 hours until 48 hours. After 48 hours the increasing number of red blood cells were observed. White blood cells were grouped into 2 types. There were granulocytes which decreased in first 24 hours and slightly increased after 24 hours. The normal percentage of granulocyte was determined within 6 days, whereas agranulocyte percentage was contrarily appeared, comparing with granulocyte. However, the 2 granulocyte percentage became normal value within 6 days.
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