Organogenesis and somatic embryogenesis in Spinacia oleracea tissue culture
1998
Zdravkovic-Korac, S. (Institute for Biological Research "Sinisa Stankovic", Belgrade (Yugoslavia)) | Neskovic, M.
Organogenesis and somatic embryogenesis were studied in spinach (Spinacia oleracea L.) callus tissue, induced in isolated leaf disks, hypocotyl and cotyledon segments. Proliferation of tissue was induced on the Murashige and Skoog (1962) medium, supplemented with 4.4 microM 2,4-dichlorophenoxy acetic acid (2,4-D) and 4.6 microM kinetin. Upon 2,4-D omission, buds were regenerated and clones with a high multiplication rate were established. The embryogenic callus appeared secondarily and was conspicuous for its red colour and embedded white embryos. The callus, producing constantly both somatic embryos and buds, was grown for up to 7 years on basal medium supplemented only with kinetic (4.6 microM). In the induction of somatic embryogenesis, kinetic was superior to benzyladenine (BA). Callus growth was promoted by GA3 and to a lesser extent by IAA, particularly in combination with BA. Both cytokinins promoted bud formation. Callus formation was positively correlated with bud initiation, and negatively with somatic embryogenesis. It is suggested that somatic embryogenesis may be fostered by possible stress effects produced by culture conditions.
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