Antibodies against bull spermatozoa - individual variability
1998
Jacevic, V. (Vojno tehnicki institut, Beograd (Yugoslavia)) | Jakovljevic, G. | Lazarevic, M.
The presence of antibodies against bull spermatozoa or against the diluent for bull semen, in blood serum and cervico-vaginal mucus of heifers and cows presents one of the main causes of asymptomatic subfertility and sterility of breeding females. With the aim of determining individual variation of the spermagglutinin titer in breeding females, in relation to the applied semen, we used 12 ejaculates from different Holstein-Friesian bulls. Cervical-vaginal mucus, blood serum of heifers and cows as well as specific control rabbit antisera (immunized with TRIS-yolk diluent in CFA with straw content for artificial insemination (a.i.) in CFA), were used for examination. A difference in the spermagglutinin titer values was observed in the studied breeding females when using semen from different bulls, but it was not pronounced (p0.05). Results indicate that the spermagglutinin titer values in blood serum and cervical-vaginal mucus were significantly higher in cows (inseminated 10 times) than in heifers (inseminated 2 times) (p0.01). By comparing the spermagglutinin titer values in blood serum among the immunized rabbits, a highly statistically significant difference was obtained (0.001) which proves that the immunization was successfully carried out. Aside from this, the level of obtained antibodies in the examined body secretions was proportional to the increase of artificial insemination (a.i.) trials.
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