The rooting and acclimatization of two fruit tree species - easy-to-root sour cherry (Prunus cerasus L.) and difficult-to-root sweet cherry (Prunus avium L.)
2000
Vasar, V. (Estonian Agricultural Univ., Tartu (Estonia))
The influence of auxin treatments on in vitro and ex vitro rooting and acclimatization of common Estonian P.cerasus L. and P.avium L. cultivars were compared. For in vitro rooting of P.cerasus, four different concentrations of indole-3-butyric acid (IBA) were tested: 0, 1, 3 and 8 mg/l. Auxins IBA and napthalene acetic acid (NAA) in concentrations of 1.5, 3, 4.5 and 6 mg/l were studied for their effect on adventitious root formation of P.avium. In ex vitro rooting of P.cerasus 3 different IBA-pretreatments were used: shoots were dipped into 3 mg/l IBA-solution for 24 h or 48 h. or planted directly in peat:sand mixture and immediately after plantingwatered with 3 mg/l IBA-solution. P.avium rooted and unrooted shoottips were planted ex vitro and watered with 3 mg/l IBA-solution as well. High rooting percentage of P.cerasus was obtained on medium containing 3 mg/l IBA. Higher auxin concentration caused callus formation and delay in root emergence. IBA was clearly superior to NAA when used to promote in vitro rooting of P.avium. Rooting ex vitro with IBA pretreatment increased the survival of P.cerasus plantlets in greenhouse. The highest number of vigorous plants was achieved by watering microshoots with IBA solution immediately after planting to peat:sand mixture. P.avium microshoots showed lower survival in greenhouse conditions than the shoots of P.cerasus. In vitro rooting generally increased the number of acclimatized sweet sherry plants
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