Regeneration of fertile doubled haploid plants from colchicine-supplemented medium in maize (Zea mays L.) anther culture

Kovacs, G.; Bohus, O.; Barnabas, B.

Regeneration of fertile doubled haploid plants from colchicine-supplemented medium in maize (Zea mays L.) anther culture

1999

Kovacs, G. | Bohus, O. | Barnabas, B.

Genome doubling is critical for obtaining fertile doubled haploid plants from maize anther culture. The most common chromosome doubling method applies colchicine to the plants. However, the conventional treatment has several disadvantages. In this experiment, anther cultures of one maize F1 hybrid were treated with 0.02% and 0.03% colchicine for three days at the beginning of microspore induction.

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