Optimization of non-radioactive AFLP [amplified fragment length polymorphism] protocol for genome analysis of important Philippine crops
1999
Balatero, C.H. | Galvez, H.F. | Padlan, C.P. | Segovia, S.H.E. | Hautea, D.M. (Philippines Univ. Los Bbanos, College, Llaguna 4031 (Philippines). Inst. of Plant Breeding)
DNA marker technologies are useful tools in plant genome analysis geared towards accelerating conventional breeding procedures. AFLP (Amplified Fragment Length Polymorphism) marker technology combines the reliability of RFLP [Restriction Fragment Length Polymorphism] and the convenience and speed of PCR-BASED marker techniques. A non-radioactive AFLP protocol using silver staining detection system was successfully tested and standardized. Silver staining detection system is relatively safe and easy to adapt under local conditions compared with the use of radioactive labelling systems. The standardized protocol developed enabled the high through-put and simultaneous detection of variation in multiple loci. Thus, the technique offers enormous practical applications in marker-assisted breeding, identification of varieties and cultivars, hybrid purity testing, quality control of seeds and other planting materials, and genetic distance analysis. In this study, the first report on the successful adaption of a non-radioactive AFLP marker protocol on a number of important Philippine crops under existing local conditions were presented. Examples of the protocol's successful application in coconut, mango, banana, abaca, tomato, potato, mungbean and corn will be shown
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