Optimization of conditions for non-radioactive microsatellite analysis of important Philippine crops
1999
Galvez, H.F. | Segovia, S.F. | Bituin, N.O. | Carcallas, R.J.U. | Alvarez, M.T.H. | Reano, C.E. | Hautea, D.M. (Philippines Univ. Los Banos, College, Laguna (Philippines). Inst. of Plant Breeding)
Microsatellite or simple-sequence repeats (SSRs) has been identified as an ideal molecular marker because of their hypervariability, abundance and uniform distribution in plant genomes. The use of radioisotopes such as 32P and 33P is the most commonly used detection system for SSRs. Under Philippine conditions, the use of radioactive detection system presents problems on waste disposal and procurement and could severely limit the widespread and routine applications of SSR technology. In this study, protocol for SSR analysis coupled with a silver staining detection technique has been developed for a number of important crops. Examples of various applications of SSR analysis in linkage map construction, genetic diversity studies and hybridity testing in coconut, corn, potato, banana and/or abaca will be presented
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