Field evaluation of Bacillus thuringiensis, insect growth regulators, chemical pesticide against Helicoverpa armigera (Huber) (Lepidoptera: Noctuidae) and their compatibility for integrated pest management
1999
Karim, S. (Punjab Univ., Lahore (Pakistan). National Centre of Excellence in Molecular Biology) | Murtaza, M. | Riazuddin, S.
A large number of Bacillus thuringiensis (Bt) isolates separated from samples collected from different ecological environments in Pakistan were characterized for Crystal Protein gene (cry) composition and pesticidal activity against a devastating polyphagous pest, Helicoverpa armigera.Isolates harboring combinations cry2A genes were found most efficacious against the target pest. One isolate designated as PR 17.4 (CEMB Bt)exhibited high levels of toxicity against H. armigera in lab bio-toxicity assays, and was therefore, chosen as a potential candidate in integrated Pest Management (IPM) strategies to control this notorious pest of valuable crops. Field efficacy of home grown cells CEMB Bt, PR 17.4, Agree (50 WP),was compared with a commercial BT formulation of Novartis, growth regulators, Match (50 EC), Insegar (25 WP), and a popular chemical pesticide, Curacron (500 EC) to investigate their effectiveness to control H. armigera in potato crops. Insegar was not significantly effective against the target pest. Locally isolated CEMB Bt was found to be as effective as the commercial Bt and chemical control agent of Novartis. A synergistic effect was also observed among, Match/Agree, CEMB Bt/Match,Match/Curacron and Agree/Curacron combinations. These studies suggest that CEMB Bt alone or in combination with other biological or chemical based pesticides can be safely recommended for pest management strategies against H. armigera with no obvious harmful effects on its predators as is the case with chemical insecticides.
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